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Volume 13, Issue 3 (Iranian Journal of Breast Disease 2020)
ijbd 2020, 13(3): 62-71 |
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Yazdanshenas N, Peeri M, Delfan M. Effect of 10 Weeks of High-Intensity Interval Training on Protein Levels of NF-kB and Expression of Atrogin-1 and MuRF-1 in Cardiomyocytes of Female Mice with Breast Cancer. ijbd 2020; 13 (3) :62-71
URL: http://ijbd.ir/article-1-830-en.html
URL: http://ijbd.ir/article-1-830-en.html
1- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, IranTehran, Iran , M.Peeri@iauctb.ac.ir
3- Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, IranTehran, Iran , M.Peeri@iauctb.ac.ir
3- Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
Abstract: (2784 Views)
Introduction: Limiting cancer-induced cardiac atrophy is a highly important for improving the survival rates and quality of life in cancer patients. The purpose of this study was to evaluate the effect of 10 weeks of high-intensity interval training (HIIT) on cardiac muscle weight, NF-kB protein expression, and expression of Atrogin-1 and MuRF-1 genes in the heart muscle of breast cancer–bearing female mice.
Methods: In this experimental study, 12 BALB/c mice with induced breast cancer were divided into control and HIIT groups. The HIIT protocol consisted of six intervals of running at 85%-90% V̇O2 peak, interspersed with 90 seconds of active rest, and five minutes of warm-up and cool-down. The left ventricular tissue was extracted 24 h following the last training session. Western blotting was used to measure protein levels of NF-kB, and quantitative real-time polymerase chain reaction was used to measure Atrogin-1 and MuRF-1 expression in the heart tissue. Data were analyzed using an independent t test at a 0.05 significance level.
Results: The mean cardiac muscle weight in the HIIT group was greater than in the control group (P < 0.001). A significant reduction in NF-kB protein levels (P = 0.004) and expression of Atrogin-1 (P < 0.001) and MuRF-1 (P < 0.001) were observed in the HIIT group when compared with control group.
Conclusion: Ten weeks of HIIT may inhibit NF-kB/Atrogin-1/MuRF-1 axis, leading to decreased atrophy and increased heart weight in mice with breast cancer.
Methods: In this experimental study, 12 BALB/c mice with induced breast cancer were divided into control and HIIT groups. The HIIT protocol consisted of six intervals of running at 85%-90% V̇O2 peak, interspersed with 90 seconds of active rest, and five minutes of warm-up and cool-down. The left ventricular tissue was extracted 24 h following the last training session. Western blotting was used to measure protein levels of NF-kB, and quantitative real-time polymerase chain reaction was used to measure Atrogin-1 and MuRF-1 expression in the heart tissue. Data were analyzed using an independent t test at a 0.05 significance level.
Results: The mean cardiac muscle weight in the HIIT group was greater than in the control group (P < 0.001). A significant reduction in NF-kB protein levels (P = 0.004) and expression of Atrogin-1 (P < 0.001) and MuRF-1 (P < 0.001) were observed in the HIIT group when compared with control group.
Conclusion: Ten weeks of HIIT may inhibit NF-kB/Atrogin-1/MuRF-1 axis, leading to decreased atrophy and increased heart weight in mice with breast cancer.
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Conclusion: Ten weeks of HIIT may inhibit NF-kB/Atrogin-1/MuRF-1 axis, leading to decreased atrophy and increased heart weight in mice with breast cancer.
Type of Study: Research |
Received: 2020/08/18 | Accepted: 2020/10/10 | Published: 2020/11/21
Received: 2020/08/18 | Accepted: 2020/10/10 | Published: 2020/11/21
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