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Volume 14, Issue 3 (Iranian Journal of Breast Diseases 2021)
ijbd 2021, 14(3): 46-56 |
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Jalali Z, Shahidi F. Interactive Effect of 6 Weeks of Aerobic Exercise and Quercetin Supplementation on TIE-2 and VEGF-A Expression in Tumor Tissue of Female Mice with Breast Cancer. ijbd 2021; 14 (3) :46-56
URL: http://ijbd.ir/article-1-900-en.html
URL: http://ijbd.ir/article-1-900-en.html
1- Ph.D. Student in Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
2- Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran ,fereshteh_shahidi98@yahoo.com
2- Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran ,
Abstract: (2345 Views)
Introduction: In recent decades, the significant role of angiogenesis in the growth and metastasis of cancer has led to much research in this field. The aim of this study was to investigate the interaction effect of 6 weeks of continuous aerobic training and quercetin on TIE-2 and VEGF-A expression in female mice with breast cancer.
Methods: Twenty-four female BALB/c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor+aerobic exercise (T+AE), and tumor+aerobic exercise+quercetin (T+AE+Q). The T+AE group and the T+AE+Q group performed endurance running exercise on a treadmill for 6 weeks, 5 days per week, 60 minutes per session, with a gradual increase in intensity over the training period. The T+AE +Q group was injected with 110 mg.kg-1 of quercetin solution for 6 weeks, /3 days per week/ in addition to exercise. Eventually, the mice were killed, and the tumor tissues were removed and frozen in liquid nitrogen. The expression of TIE-2 and VEGF-A genes was measured using real-time PCR. ΔCt, ΔΔCt, and the fold change were calculated, and one-way analyses of variance with Tukey post hoc tests were used to analyze the data at a significance level of 0.05. Data analysis was conducted using the GenEx software.
Results: The results showed that T+AE+Q interaction significantly reduced VEGF-A expression (4.09 times decrease) compared with the T group (P< 0.05). Quercetin consumption in the T+AE+Q group significantly reduced VEGF-A expression (2.72 times) compared with the T+AE group (P< 0.05)., However, aerobic exercise alone had no effect on VEGF-A expression. Also, aerobic exercise alone or in combination with quercetin had no effect on TIE-2 expression.
Conclusion: The interaction of aerobic exercise and quercetin supplementation may be effective in inhibiting tumor angiogenesis.
Methods: Twenty-four female BALB/c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor+aerobic exercise (T+AE), and tumor+aerobic exercise+quercetin (T+AE+Q). The T+AE group and the T+AE+Q group performed endurance running exercise on a treadmill for 6 weeks, 5 days per week, 60 minutes per session, with a gradual increase in intensity over the training period. The T+AE +Q group was injected with 110 mg.kg-1 of quercetin solution for 6 weeks, /3 days per week/ in addition to exercise. Eventually, the mice were killed, and the tumor tissues were removed and frozen in liquid nitrogen. The expression of TIE-2 and VEGF-A genes was measured using real-time PCR. ΔCt, ΔΔCt, and the fold change were calculated, and one-way analyses of variance with Tukey post hoc tests were used to analyze the data at a significance level of 0.05. Data analysis was conducted using the GenEx software.
Results: The results showed that T+AE+Q interaction significantly reduced VEGF-A expression (4.09 times decrease) compared with the T group (P< 0.05). Quercetin consumption in the T+AE+Q group significantly reduced VEGF-A expression (2.72 times) compared with the T+AE group (P< 0.05)., However, aerobic exercise alone had no effect on VEGF-A expression. Also, aerobic exercise alone or in combination with quercetin had no effect on TIE-2 expression.
Conclusion: The interaction of aerobic exercise and quercetin supplementation may be effective in inhibiting tumor angiogenesis.
Keywords: Breast Cancer, Aerobic Exercise, Quercetin, TIE-2 Receptor, Vascular Endothelial Growth Factor
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Conclusion: The interaction of aerobic exercise and quercetin supplementation may be effective in inhibiting tumor angiogenesis.
Type of Study: Research |
Received: 2021/05/19 | Accepted: 2021/07/18 | Published: 2021/10/23
Received: 2021/05/19 | Accepted: 2021/07/18 | Published: 2021/10/23
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