Shaghayegh Vojdani Nejad Yazdi, Hadi Zare-Zardini, Seyed Mohsen Miresmaeili, Farzaneh Fesahat,
Volume 15, Issue 1 (4-2022)
Abstract
Introduction: The use of drug delivery systems can increase the effectiveness of chemotherapy and reduce its side effects in the treatment of breast cancer. This study aimed to evaluate the effect of doxorubicin-containing silk fibroin nanoparticles (NF-DOX) on P53 gene expression in breast cancer cell lines and to measure its toxicity in vitro.
Methods: NF-DOX was synthesized and characterized. The breast cancer cell line MCF-7 and normal HFF cell line were treated with different concentrations of NF-DOX, and its toxicity and relative expression of P53 were measured.
Results: Examination and characterization of the synthesized compound of NF-DOX indicated its nanometer dimension. The drug’s cytotoxic effect on MCF-7 cells was significantly greater compared with HFF cells and the control group (P < 0.001). The IC50 values (half-maximal inhibitory concentration) for MCF-7 and HFF cells treated with NF-DOX were 229 and 647 µg/ml, respectively. The relative gene expression levels of P53 in MCF-7 and HFF cell lines compared with the controls were measured as 27.09 ± 0.51 and 0.57 ± 0.07, respectively. The relative gene expression of P53 in MCF-7 and HFF cell lines showed a significant increase (P < 0.0001) and decrease (P < 0.0006) compared with controls, respectively.
Conclusion: Differential and significant changes in P53 gene expression in cell lines indicate that NF‑DOX may play an effective role in inhibition of breast cancer metastasis. The results showed a dose- and time-dependent anticancer effect of NF‑DOX, and it can be considered as a candidate for a new anticancer drug.
Solmaz Sadeghian, Yaser Kazemzadeh, Yahya Mohammadnejadpanah Kandi, Sanaz Mirzayan Shanjani, Saeed Sedaghati,
Volume 15, Issue 4 (1-2023)
Abstract
Introduction: Chemotherapy for the treatment of breast cancer has many side effects on the liver tissue. The aim of the present study was to investigate the effect of aerobic exercise and curcumin on oxidative stress indices in the liver of mice with cancer treated with doxorubicin.
Methods: The present study is basic and experimental. Thirty-six female BALB/c mice weighing 20 ± 2 g were randomly divided into 6 groups: healthy, patient, patient + treatment (doxorubicin), patient + treatment + training, patient + treatment + supplement, and patient + treatment + supplement + aerobic exercise. Cancer was induced by the injection of the 4T1 cell line. Doxorubicin was injected subcutaneously on days 1, 7, 14, 21, and 28, and curcumin was given orally. Aerobic exercise consisted of 30 minutes of running at an intensity of 40%-60% of the maximum speed, 5 days a week, for 6 weeks. The training started at 14 m/min in the first week and finally reached 18 m/min. Two-way analysis of variance was used to investigate the interactive effect of exercise and supplementation on the expression of target genes.
Results: The interactive effect of curcumin and aerobic exercise on the expression of GSH (P = 0.054), SOD (P = 0.145), and CAT (P = 1.000) genes and concentration of MDA (P = 0.087) was not statistically significant.
Conclusion: It seems that aerobic exercise with curcumin may not have protective effects on the liver against oxidative stress caused by the drug; however, more studies are needed in this area.
