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Volume 19, Issue 2 (Iranian Journal of Breast Diseases 2026)
ijbd 2026, 19(2): 4-17 |
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Ethics code: IR.IAU.KERMAN.REC.1400.015
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Dabaghi S, Hajirezaei M. Detection frequency of BLV Tax gene in human blood and breast tissue samples using Nested-PCR. ijbd 2026; 19 (2) :4-17
URL: http://ijbd.ir/article-1-1207-en.html
URL: http://ijbd.ir/article-1-1207-en.html
1- Department of Microbiology, Islamic Azad University, Kerman, Iran
2- Department of Microbiology, Islamic Azad University, Kerman, Iran ,ma.hajirezaei@gmail.com
2- Department of Microbiology, Islamic Azad University, Kerman, Iran ,
Abstract: (832 Views)
Introduction: Many viruses have been associated with human cancers, and approximately 12% of cancers are believed to have a viral cause. One of the newest of these viruses is the bovine leukemia virus(BLV), which was first reported in 2007 in women with breast cancer. Several studies have been conducted on paraffin-embedded breast tissue samples, and a smaller number on blood samples from women, to detect this virus. This study aims to describe the frequency of the Tax gene of the BLV virus in blood samples and paraffin-embedded human breast tissue using the nested-PCR method.
Methods: In this study, which was conducted in 2022, the presence of bovine leukemia virus based on the Tax gene was investigated in 100 random paraffin-embedded breast tissue samples (50 patient samples and 50 healthy samples) and 120 random blood samples (60 patient samples and 60 healthy samples) using nested polymerase chain reaction. Data analysis was performed using SPSS version 26 software and Fisher’s exact test.
Results: In the present study, the frequency of this virus in paraffin-embedded breast tissue samples was 1 case (1%), and in blood samples from female subjects, 18 cases (15%).
Conclusion: Despite the apparent frequency difference between blood samples and paraffin-embedded tissue, it was statistically close to the significant level(P=0.057). And since blood sampling is a more non-invasive method and the incidence of breast cancer in Iran is very high, these results can be used as an introduction to conducting larger, longitudinal studies to design molecular screening systems for at-risk groups.
Methods: In this study, which was conducted in 2022, the presence of bovine leukemia virus based on the Tax gene was investigated in 100 random paraffin-embedded breast tissue samples (50 patient samples and 50 healthy samples) and 120 random blood samples (60 patient samples and 60 healthy samples) using nested polymerase chain reaction. Data analysis was performed using SPSS version 26 software and Fisher’s exact test.
Results: In the present study, the frequency of this virus in paraffin-embedded breast tissue samples was 1 case (1%), and in blood samples from female subjects, 18 cases (15%).
Conclusion: Despite the apparent frequency difference between blood samples and paraffin-embedded tissue, it was statistically close to the significant level(P=0.057). And since blood sampling is a more non-invasive method and the incidence of breast cancer in Iran is very high, these results can be used as an introduction to conducting larger, longitudinal studies to design molecular screening systems for at-risk groups.
Type of Study: Research |
Subject:
molecular cell
Received: 2025/09/18 | Accepted: 2026/02/21 | Published: 2026/07/26
Received: 2025/09/18 | Accepted: 2026/02/21 | Published: 2026/07/26
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