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Volume 19, Issue 3 (Iranian Journal of Breast Diseases 2026)
ijbd 2026, 19(3): 37-51 |
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Ethics code: IR.AJUMS.HGOLESTAN.REC.1404.038
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Farzaneh M, Miri H, Cheraghzadeh M, Sasani N, Sasani M. Investigating the Effect of the Supernatant of Mesenchymal Stem Cells (MSCs) Derived from the Lung Tissue of Chicken Embryo on the Expression Levels of GLI2 and WNT4 Growth-Regulatory Genes in the Breast Cancer Cell Line (MCF-7). ijbd 2026; 19 (3) :37-51
URL: http://ijbd.ir/article-1-1234-en.html
URL: http://ijbd.ir/article-1-1234-en.html
1- Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Department of Basic Sciences, Faculty of Veterinary Medicine, Chamran University of Ahvaz, Ahvaz, Iran
5- Department of Emergency Medicine, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Cancer, Environmental and Petroleum Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ,sasani-m@ajums.ac.ir
2- Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Department of Basic Sciences, Faculty of Veterinary Medicine, Chamran University of Ahvaz, Ahvaz, Iran
5- Department of Emergency Medicine, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Cancer, Environmental and Petroleum Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ,
Abstract: (252 Views)
Introduction: Mesenchymal stem cells (MSCs) can modulate the tumor microenvironment and influence cancer cell behavior by secreting various bioactive factors. This study aimed to investigate the effect of MSC-derived secretions from chick embryo lung tissue on the expression of the tumorigenic genes WNT4 and GLI2 and of inflammatory factors in a human breast cancer cell line (MCF-7).
Materials and Methods: In this in vitro experimental study, cells were isolated and cultured from 10-day-old chick embryo lung tissue. In the third passage, the supernatant (CM; conditioned medium) obtained from MSCs was collected, and its effect on MCF-7 cells was examined. After 48 hours, CM was collected and filtered. ELISA measured the levels of the inflammatory cytokines IL-6 and TNF-α, and qRT-PCR measured the levels of the WNT4 and GLI2 genes. For statistical comparisons between groups, an independent t-test was used. All analyses were performed using SPSS version 26, and 0.05 was considered the significance threshold.
Results: Treatment of MCF-7 cells with MSCs-CM resulted in a significant decrease in the expression of WNT4 (P=0.0034) and GLI2 (P=0.0007) genes in the treatment group compared to the control group. Also, the levels of IL-6 (P=0.0155) and TNF-α (P=0.0073) in the treatment group showed significant decreases.
Conclusion: MSCs-CM can exert inhibitory effects on cancer cells by downregulating key genes in tumorigenic signaling pathways and reducing inflammatory factors.
Materials and Methods: In this in vitro experimental study, cells were isolated and cultured from 10-day-old chick embryo lung tissue. In the third passage, the supernatant (CM; conditioned medium) obtained from MSCs was collected, and its effect on MCF-7 cells was examined. After 48 hours, CM was collected and filtered. ELISA measured the levels of the inflammatory cytokines IL-6 and TNF-α, and qRT-PCR measured the levels of the WNT4 and GLI2 genes. For statistical comparisons between groups, an independent t-test was used. All analyses were performed using SPSS version 26, and 0.05 was considered the significance threshold.
Results: Treatment of MCF-7 cells with MSCs-CM resulted in a significant decrease in the expression of WNT4 (P=0.0034) and GLI2 (P=0.0007) genes in the treatment group compared to the control group. Also, the levels of IL-6 (P=0.0155) and TNF-α (P=0.0073) in the treatment group showed significant decreases.
Conclusion: MSCs-CM can exert inhibitory effects on cancer cells by downregulating key genes in tumorigenic signaling pathways and reducing inflammatory factors.
Type of Study: Research |
Subject:
molecular cell
Received: 2026/02/12 | Accepted: 2026/07/26 | Published: 2026/10/22
Received: 2026/02/12 | Accepted: 2026/07/26 | Published: 2026/10/22
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