Enter your email address
Submit
Write your message
Volume 11, Issue 1 (Iranian Quarterly Journal of Breast Disease 2018)
ijbd 2018, 11(1): 37-46 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ahmadian M, Azizbeigi K, Delphan M, Atashak S. The Effect of High Intensity Interval Training on STAT-3 and Angiopoietin-1 Gene Expression, and tie-2 Protein in Mice with Breast Cancer. ijbd 2018; 11 (1) :37-46
URL: http://ijbd.ir/article-1-657-en.html
URL: http://ijbd.ir/article-1-657-en.html
1- Physical Education Department, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2- Physical Education Department, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , kazizbeigi@gmail.com
3- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran
4- Physical Education Department, Mahabad Branch, Islamic Azad University, Mahabad, Iran
2- Physical Education Department, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , kazizbeigi@gmail.com
3- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran
4- Physical Education Department, Mahabad Branch, Islamic Azad University, Mahabad, Iran
Abstract: (4925 Views)
Introduction: Angiogenesis has a great role in physiological development of tissues and tumors. Considering the key role of STAT-3 and angiopoietin-1-tie-2 in angiogenesis, this study aimed to investigate the effect of 10 weeks of high intensity interval exercise (HIIT) on STAT-3 and angiopoietin-1 gene expression, and tie-2 protein in mice with breast cancer.
Methods: After inducing cancer (subcutaneous injection of MC4-L2 on the right side of mice), twelve Balb/c mice (6-8 weeks, weight 19±1.05) were randomly assigned to HIIT (n=6) and non-active groups (n=6). HIIT sessions were performed five days a week for 10 weeks. Twenty-four hours after the last session of HIIT, the animals were sacrificed and the tumor tissue was isolated for measuring the expression of STAT-3 and angiopoietin-1 gene by qreal time PCR and measuring tie-2 protein by western blot method. Tumor volume changes were also assessed using a digital caliper every week.
Results: The results of this study showed a significant decrease in expression of STAT-3 and angiopoietin-1, and changes in tumor volume and tie-2 in the training group compared to the control group (p =0.001).
Conclusion: It appears that high intensity interval exercise can reduce the rate of tumor progression through decreasing the expression of some angiogenesis promoters, and is an effective non-medical method for reducing tumor growth.
Methods: After inducing cancer (subcutaneous injection of MC4-L2 on the right side of mice), twelve Balb/c mice (6-8 weeks, weight 19±1.05) were randomly assigned to HIIT (n=6) and non-active groups (n=6). HIIT sessions were performed five days a week for 10 weeks. Twenty-four hours after the last session of HIIT, the animals were sacrificed and the tumor tissue was isolated for measuring the expression of STAT-3 and angiopoietin-1 gene by qreal time PCR and measuring tie-2 protein by western blot method. Tumor volume changes were also assessed using a digital caliper every week.
Results: The results of this study showed a significant decrease in expression of STAT-3 and angiopoietin-1, and changes in tumor volume and tie-2 in the training group compared to the control group (p =0.001).
Conclusion: It appears that high intensity interval exercise can reduce the rate of tumor progression through decreasing the expression of some angiogenesis promoters, and is an effective non-medical method for reducing tumor growth.
Keywords: Breast Cancer, High Intensity Interval Training, Angiogenesis, STAT-3, Angiopoietin-1 and tie-2
Full-Text [PDF 112 kb]
(1998 Downloads)
It appears that high intensity interval exercise can reduce the rate of tumor progression through decreasing the expression of some angiogenesis promoters, and is an effective non-medical method for reducing tumor growth.
Type of Study: Research |
Subject:
Breast Diseases
Received: 2018/01/25 | Accepted: 2018/06/26 | Published: 2018/06/26
Received: 2018/01/25 | Accepted: 2018/06/26 | Published: 2018/06/26
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
