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Volume 16, Issue 1 (Iranian Journal of Breast Diseases 2023)
ijbd 2023, 16(1): 49-64 |
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bazsefidpar P, Eftekhar E, Nikpoor A R, Zolghadri S, Zareian Jahromi M. Toxicity effect of synthesized platinum Schiff bases on SKBR3 breast cancer cell line. ijbd 2023; 16 (1) :49-64
URL: http://ijbd.ir/article-1-997-en.html
URL: http://ijbd.ir/article-1-997-en.html
Parisa Bazsefidpar1 
, Ebrahim Eftekhar2 , Amin Reza Nikpoor2 , Samaneh Zolghadri1 , Mohammad Zareian Jahromi * 3
, Ebrahim Eftekhar2 , Amin Reza Nikpoor2 , Samaneh Zolghadri1 , Mohammad Zareian Jahromi * 3
1- Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2- Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3- Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran ,mzareian88@gmail.com
2- Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3- Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran ,
Abstract: (1119 Views)
Introduction: Breast cancer is a prevalent disease that has not been entirely treated by chemotherapy. This study attempted to investigate the effect of the cytotoxicity of Schiff bases derived from platinum (II) on the breast cancer cell line (SKBR3) and the expression of genes involving molecular pathways.
Materials and Methods: In this study, at first, the cytotoxicity of carboplatin (as the standard drug) and Schiff base derived from platinum (II) (12.5, 52, and 50 µM) on the SKBR3 cell line was investigated using MTT assay, and the half maximal inhibitory concentration (IC50) was determined. After cell treatment, RNA was extracted, and cDNA was synthesized. Then gene expression of various cellular pathways of apoptosis (BCL2 and BAX) and autophagy (ATG5, BECN1, TOP1, LC3, and HIF1A) was evaluated using the Real-time PCR method.
Results: Cell treatment with platinum (II) Schiff bases complexes in various concentrations showed that complex A (IC50=34.76±2.16µM) had the most significant toxicity compared to other studied compounds. The synthesized complexes may affect the cytotoxicity of SKBR3 through suppression of HIF1A and TOP1 genes. Statistical analysis of samples was conducted using SPSS software program, two-way ANOVA analysis, and Kruskal-Wallis tests at the significance level of 0.05 and 0.001.
Conclusion: In this research, platinum-based Schiff bases showed a significant cytotoxic effect against the SKBR3 cell line. More information in this field can pave the way for discovering anticancer platinum drugs with more favorable properties.
Materials and Methods: In this study, at first, the cytotoxicity of carboplatin (as the standard drug) and Schiff base derived from platinum (II) (12.5, 52, and 50 µM) on the SKBR3 cell line was investigated using MTT assay, and the half maximal inhibitory concentration (IC50) was determined. After cell treatment, RNA was extracted, and cDNA was synthesized. Then gene expression of various cellular pathways of apoptosis (BCL2 and BAX) and autophagy (ATG5, BECN1, TOP1, LC3, and HIF1A) was evaluated using the Real-time PCR method.
Results: Cell treatment with platinum (II) Schiff bases complexes in various concentrations showed that complex A (IC50=34.76±2.16µM) had the most significant toxicity compared to other studied compounds. The synthesized complexes may affect the cytotoxicity of SKBR3 through suppression of HIF1A and TOP1 genes. Statistical analysis of samples was conducted using SPSS software program, two-way ANOVA analysis, and Kruskal-Wallis tests at the significance level of 0.05 and 0.001.
Conclusion: In this research, platinum-based Schiff bases showed a significant cytotoxic effect against the SKBR3 cell line. More information in this field can pave the way for discovering anticancer platinum drugs with more favorable properties.
Type of Study: Research |
Received: 2022/07/17 | Accepted: 2023/03/1 | Published: 2023/04/19
Received: 2022/07/17 | Accepted: 2023/03/1 | Published: 2023/04/19
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